CircPrime: a web-based platform for design of specific circular RNA primers

CircRNA coordinates:

Input file (output of the bioinformatic predictor of circRNAs or BED file)

The BED file must contain the first 4 fields (chrom, chromStart, chromEnd and name)

Reference Genome

shiftA

Shift range left

shiftB

Shift range right

PRIMER_PRODUCT_SIZE_RANGE

PCR product size

PRIMER_OPT_SIZE

Optimum length of a primer

PRIMER_MIN_SIZE

Minimum acceptable length of a primer

PRIMER_MAX_SIZE

Maximum acceptable length of a primer

PRIMER_OPT_TM

Optimum melting temperature for a primer in Celsius

PRIMER_MAX_TM

Maximum acceptable melting temperature for a primer in Celsius

PRIMER_MIN_TM

Minimum acceptable melting temperature for a primer in Celsius